Raw Immunofluorescence Images and Quantification Data of Testicular Cells in Rhesus Macaques Following Monkeypox Virus (MPXV) Infection

This dataset contains the original immunofluorescence microscopy images and quantitative analysis results used in the study “Analysis of pathological changes and virus-susceptible cells in the testes following MPXV infection in a rhesus macaque model.”The dataset includes raw fluorescence images and corresponding quantitative measurements obtained from testicular tissue sections of rhesus macaques collected during the acute phase of Monkeypox virus (MPXV) infection (5 days post-infection). Immunofluorescence staining was performed to evaluate viral distribution, cellular tropism, structural proteins, immune responses, and testicular cell populations.The images include staining for MPXV antigen and multiple cellular markers representing major testicular cell types and functional pathways, including:Sertoli cells (SOX9, Vimentin)Spermatogonia (SALL4)Spermatocytes (GKAP1)Cell cycle marker (CCNB2)Tight junction proteins associated with the blood–testis barrier (ZO-1, Occludin)Leydig cells (HSD3B1)Vascular endothelial cells (CD31)Immune cells including macrophages (CD68), CD4⁺ T cells, CD8⁺ T cells, and B cells (CD19)Complement component (C1q)Apoptosis marker (Caspase-3)Interferon pathway factor (IRF3)The dataset also contains quantitative results used for statistical analyses in the manuscript, including:Cell counts of marker-positive cellsPercentage of marker-positive areaCo-localization analysis between MPXV antigen and host cellular markersData used for generating figures and statistical comparisonsAll raw numerical data underlying the figures in the manuscript are provided to ensure transparency and reproducibility. Images are provided in their original format as obtained from fluorescence microscopy.