Gene expression profiles in lens from KLPH/lctl knockout mice versus wild type controls by RNA-seq

Lenses from 6 week old KLPH/lctl knockout mice versus wild type controls were used to prepare total RNA for RNA-sequencing. Differential mRNA expression between KLPH/lctl knockout mice and age matched C57bl6/J control mice was quantified by RNA-seq data analysis using the Tuxedo package (i.e. the Tophat-Cufflinks pipeline) Differential mRNA expression between KLPH/lctl knockout mice and age matched C57bl6/J control mice was quantified by RNA-seq data analysis本研究以6周龄KLPH/lctl基因敲除（knockout）小鼠与野生型对照小鼠的晶状体为实验材料，提取总RNA用于RNA测序（RNA-sequencing）。采用Tuxedo套件（即Tophat-Cufflinks分析流程）开展RNA测序数据分析，定量分析KLPH/lctl基因敲除小鼠与同周龄C57bl6/J对照小鼠的mRNA差异表达水平。通过RNA测序数据分析，完成KLPH/lctl基因敲除小鼠与同周龄C57bl6/J对照小鼠的mRNA差异表达定量分析。