LipidIN: a comprehensive repository for flash platform-independent identification and reverse lipidomics

We present expeditious querying, reliable and platform-independent lipid annotation software LipidIN, a comprehensive lipidome atlas with tandem mass spectrometry information, relative retention time and reverse mass spectrometry information. Five-levels spectral fragmentations trees were formulates across 165.6 million lipids with Paternò − Büchi reaction and Electron activated dissociation included. Flash entropy search speeds up 69.65 billion times spectra querying in less than 1 s. Lipid categories intelligence models and reverse mass spectrometry information dramatically reduce false discoveries. Using 1833 clinical cohorts to demonstrate the reliability of lipidIN in clinical lipid investigations.