Ikaros/STAT5 DNA binding competition in pre-B cell line [ChIP-seq]. Ikaros/STAT5 DNA binding competition in pre-B cell line [ChIP-seq]

Ikaros was found to antagonize the transcriptional program of IL-7 activated pathways during pre-B cell differentiation. We analyzed the genomic distribution of Ikaros and STAT5 (downstream effector of IL-7; in the presence or absence of Ikaros) in pre-B cells by ChIP-Seq. Overall design: A Ikaros deficient, IL-7 dependent pre-B cell line was reconstituted with a Tamoxifen inducible Ikaros-ER fusion protein. 24h after Ikaros induced expression, we performed an Ikaros and STAT5 ChIP-Seq analysis. The samples Ikaros-ChIP and Input are re-used from GSE114629.