Transcriptome-wide binding profile of RNA-binding protein HuR in MDA-MB-231 cells

The purpose of this study is to generate RNA binding profile of RNA-binding protein HuR in human breast cancer cell line MDA-MB-231 cells by ribonucleoprotein immunoprecipitation sequencing (RIP-deq). RNA immunoprecipitated by mouse anti-HuR antibody or mouse IgG was collected for library prepareation and deep sequencing, in duplicate, using Illumina Hiseq 2500 system. About 30 million sequence reads per sample were mapped to the human genome (build hg38), the corresponding peaks were then detected and annotated. Finally, the RIP peaks that correspond to significant transcript abundance change were identified and compiled. Overall design: RNA binding profile of RNA-binding protein HuR in MDA-MB-231 cells was generated by RIP-seq, in duplicate, using Illumina Hiseq 2500 system.