Response in Shewanella oneidensis MR-1 and so2426 deletion mutant to Hexavalent Chromium over a 24 hour period

Shewanella oneidensis MR-1 exhibits diverse metal ion-reducing capabilities and thus is of potential utility as a bioremediation agent. Knowledge of the molecular components and regulatory mechanisms dictating cellular responses to heavy metal stress, however, remains incomplete. In a previous work, the S. oneidensis so2426 gene, annotated as a DNA-binding response regulator, was demonstrated to be specifically responsive at both the transcript and protein levels to acute chromate [Cr(VI)] challenge. To delineate the cellular function of SO2426 and its contribution to metal stress response, we integrated genetic and physiological approaches with a genome-wide screen for target gene candidates comprising the SO2426 regulon. Keywords: Time course At each timepoint (post chromate shock) samples were collected from WT and mutant which were grown in biological triplicates. Final number was 6 samples per time point.