Best Practices for the Collection of Robust Time Course Reaction Profiles for Kinetic Studies

Kinetic investigations can provide critical mechanistic
information
for the optimization of the reaction parameters and reaction development.
Modern kinetic analyses such as RPKA and VTNA provide many advantages
over traditional initial rate methods and are especially powerful
when coupled with reaction monitoring technologies. While these are
robust analytical methods, the lack of careful observation and optimization
can lead to misinterpretation of the data. In this Perspective, we
highlight some commonly overlooked considerations in kinetic studies
based on our experiences and present a general guide to proper optimization
of reactions and analytics prior to acquiring kinetic data.