Data underlying the research on particle manipulation using hydrodynamic forcing. This dataset consist of the experimental data of particle interchanging its position in a Hele-Shaw cell.

The reseach objective is to present a microfluidic approach to achieve the dynamic control of particle pathlines within a flow through microfluidic device. Our approach combines three key aspects: the design of a flow-through microfluidic flow cell with the ability to manipulate the streamlines of the flow, an optimization procedure to find a priori optimal particle path-lines, and a Proportion-Integral-Derivative-based (PID) feedback controller to provide real time control over the particle manipulations. The experimental raw images were recorded with a sCMOS camera (PCO) with a pixel pitch of 6.5 μm. The camera was mounted on a microscope (Nikon Eclipse Ti) with a 1x objective. The<br> acquisition frequency was 5 Hz corresponding to an average in-plane displacement of 4-6 pixels between two consecutive recordings. The zip file contains the raw images and the MATLAB script used to do an experiment of two particles interchanging their positions by only using the hydrodynamic forcing in a Hele-Shaw cell.

本研究旨在提出一种微流控方案，实现微流控装置内流体中粒子运动迹线的动态调控。
本方案融合三大核心要素：一是设计具备流体流线调控能力的流通式微流控流池；二是构建优化流程以求解先验最优粒子运动迹线；三是采用基于比例-积分-微分（Proportion-Integral-Derivative, PID）的反馈控制器，实现粒子调控的实时控制。
实验原始图像由sCMOS相机（PCO）采集，该相机的像素间距为6.5 μm。
该相机搭载于配备1×物镜的尼康（Nikon）Eclipse Ti显微镜之上。采集频率为5 Hz，对应两次连续采集间的平面内平均位移为4至6个像素。
本压缩包包含原始图像，以及用于开展Hele-Shaw流池内仅通过流体动力调控实现两枚粒子位置互换实验的MATLAB脚本。