Gene expression in human keratinocytes cultivated with serum-free medium and serum containing medium

The objective of this study was thus to develop ab initio that chemically defined serum-free medium. We used a statistical design of experiment (DOE) approach to screen a large number of candidate factors, for both their potential individual and synergetic effects, as to identify a formulation which was then tested extensively. In this study, we present the properties of keratinocyte cultivated with serum-Free medium (Surge SFM) and complemented DME-Ham medium (with serum). Four human keratinocyte populations at passage 2 cultivated with with serum-free medium or serum containing medium until cells attained 80-95% confluence.