RSV and FIDAS down-regulate activity of AR-dependent reporter-gene activity in LNCaP and 22Rv1 cells.

AR-positive LNCaP and 22Rv1 cells were transfected with the reporter-gene plasmids pARE(2x)-luc and pRT-TK, as described in Material and Methods. Subsequently cells were incubated with RSV or FIDAS in presence/absence of 5 nM DHT for 24 hours. AR-reporter-gene activity was determined using the Dual-Luciferase Reporter Assay System (Promega) according to the manufactures instructions. Results are expressed in % (ARDHT stimulated/ARbasal activity) which was set at 100% for RSV or FIDAS untreated cells (controls). Each value represents the mean of at least 3 independent experiments ± standard deviation, (p-values compared to corresponding DHT-treated control *p<0.01, **p<0.05)