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20230911 PFEV miRNA datasets

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Research Data Australia2024-08-03 收录
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https://researchdata.edu.au/20230911-pfev-mirna-datasets/3341797
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The Nanostring nCounter® Human v3 miRNA expression assay panel includes 798 miRNA probes to screen 827 miRNAs that are highly curated in miRBase 22 (released 12th March 2018). Unique oligonucleotide tags were ligated onto native miRNAs in a total RNA sample, followed by hybridisation (12 – 30 hours) of tagged miRNAs and capture/reporter probes with colour-coded sequence specific to each miRNA target. Post-hybridisation samples were then loaded onto a SPRINT cartridge, and processed on the Nanostring nCounter® SPRINT Profiler (NanaString Technologies, Seattle, WA, USA). Data was acquired through instrument automated fluorescence microscope scanning with direct counts of target-probe complexes, and analysed by Nanostring nSolver™ software.
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The University of Queensland
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