Single cell profiling of SFB induced transcriptomic changes in small intestinal epithelium and immune system.

In this project we profiled small intestinal epithelium, lamina propria immune cells as well as intraepithelial immune cells from 5-weeks old WT mice derived from Jackson laboratories and littermate colonized with segmented filamentous bacteria (SFB) 2 weeks prior to analysis, using 10x droplet-based single-cell RNA sequencing.本研究采用10×液滴式单细胞RNA测序技术，对两类小鼠的小肠上皮、固有层免疫细胞及上皮内免疫细胞进行了单细胞转录组特征分析：一类为源自杰克逊实验室的5周龄野生型（WT）小鼠，另一类为分析前2周定植了分段丝状细菌（SFB）的同窝仔鼠。