soil metagenome Raw sequence reads. soil metagenome

The fertilization practices are increasingly recognized as a factor influencing the soil microbial community structure furthermore microorganisms are critical to maintaining soil fertility by virtue of their ecological role in ecosystem services. Generally, microbial communities react to changing in the fertilization practices and at environmental conditions. It’s known that vineyard harbors a specific set of microorganisms. The microbial ecology of the vineyard has been extensively investigated to decipher the associated microbial populations as well as the dynamics of microorganisms in wine fermentation. Despite the pivotal role of soil microbiome, the awareness that different land uses can strongly influence the soil microbial community dynamics and abundances affecting the soil biodiversity, an interesting still open question is how the fertilization practice influences the soil microbiome. In this context, establishing the hypothesis that bacterial community structure would be defined by the different agronomic practices, the goal of this work is to evaluate the long-term effects of two fertilization practices (Mineral, MD and Organo-Mineral, OMD) on soil bacterial community. In particular, a massive DNA sequencing was done on soil samples, using an Illumina platform, to amplify the hypervariable region V3-V4 of the 16S rRNA gene. The results allowed to speculate how fertilization quality has a potential impact in driving the shape and the composition of soil bacterial communities. diversity was highly variable over time harboring the most diverse soil bacterial communities changing on a year basis. In contrast, -diversity patterns are relatively constant and moderate changes are occurred and selected by fertilization practices. In total 23 classes were identified with a relative abundance higher than 1 %. A t-test applied on the OTUs displayed significant differences in abundance (p < 0.05) between treated soils (MD, OMD) and the control (C). In the OMD treated samples, respect to the control, the classes of Acidobacteria-6, Nitrospira, Phycisphaerae, Betaproteobacteria, Alphaproteobacteria, Spartobacteria appeared to be suppressed. The comparison between the Mineral treated soil samples and the control shown that classes of Nitrospira, BPC102, Phycisphaerae, Planctomycetia are suppressed whereas Chloracidobacteria, Alphaproteobacteria, Betaproteobacteria (MD) seemed to be stimulated. In conclusion, we feel the need to recognize the pivotal role of substrate and nutrient utilization ability and efficiency in driving the magnitude and the effect on soil microbial community response.