Mobilome, on-farm biopurification system Kortrijk

Biopurification system (BPS) are used to treat pesticide wastewater streams on farmyards. The matrix consists of a mixture of agricultural soil and straw and by the pesticide application these environments are highly enriched in pesticides. BPS matrix samples originating from the BPS in Kortrijk were described before (Dealtry, Holmsgaard, et al., 2014), aiming to study the seasonal changes in the pool of MGE content as well as the pesticide compounds that were loaded on the BPS matrix. Samples were collected before start-up in March and during the spraying season in July and September of the agricultural season of 2011. An additional sampling taken just after the seasonal shut down of the BPS in December 2011, is included in this study. Here by a novel metagenomic tool, the accessory genes of the IS1071 composite transposons and IncP-1 plasmids was targeted by means of a long range PCR method. Aiming to further study the ecological significance of these mobile genetic elements in pesticide contaminated environments. Dealtry S, Holmsgaard PN, Dunon V, et al. (2014) Shifts in abundance and diversity of mobile genetic elements to diverse pesticides introduced into an on-farm biopurification system over a year.