RNA-Seq Analysis of Diuraphis noxia biotypes 1 and 2 feeding on Dn4 and Dn0 wheat cultivars

Age-synchronized biotype 1 and 2 aphids were allowed to feed on wheat cultivars containing Dn0 and Dn4 resistance genes for 24 hours for a total of four different treatments: biotype 1 on Dn0 and Dn4 plants and biotype 2 on Dn0 and Dn4 plants. After aphids in each treatment had fed for 24h, groups of 30 aphids were collected from each of the three biological replicates of each treatment group, randomly pooled to account for variation in rearing conditions. Approximately 40 million reads were generated from each sample using an Illumina NovaSeq.

本实验中，同步饲养的生物型1和生物型2的蚜虫被允许在含有Dn0和Dn4抗性基因的小麦品种上取食24小时，共进行四种不同的处理：生物型1在Dn0和Dn4植株上，生物型2也在Dn0和Dn4植株上。在每个处理组中，蚜虫取食24小时后，从每个处理组的三个生物重复中收集了30只蚜虫，随机混合以考虑饲养条件中的变化。每个样本通过Illumina NovaSeq生成了约4000万个读数。