Comprehensive transcriptomic analysis of immune-related genes in diabetic foot ulcers: New insights into mechanisms and therapeutic targets

We performed RNA-seq on tissue biopsies derived from patients with DFU and compared it to healthy controls who had similar foot surgery to identify the significant immune related differentially expressed genes between normal and DFU samples. Our results identified that there was a total of 8800 DEGs detected by RNA-seq data analysis, among which 2351 were upregulated and 6449 downregulated genes in DFU. 526 differential IRGs were obtained from intersection of DEGs and IRGs. These results suggest that a deregulated immune cells contribute to pathogenesis of DFUs.The purpose of this study was to identify the key biomarkers of the abnormally expressed genes and immune infiltration in the diabetic wound and to provide diagnostic and therapeutic targets for DFU. Overall design: mRNA profiles of diabetic foot ulcers patients and healthy controls.