Optimized CRISPR-Cas12a platform for combinatorial drop-out genetic screening (RNA-seq)
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下载链接：
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE141130
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资源简介：
Combinatorial genetic perturbations have been utilized to identify synthetic sick/lethal genetic interactions for cancer drug target discovery. Current methods for high-throughput combinatorial genetic screening are inefficient and cumbersome. Here we developed a simple, robust, and high-performance CRISPR-Cas12a-based approach for unbiased, combinatorial genetic screening in cancer cells. mRNA profiles of Cas12 sgRNA-expressing MLL-AF9 transformed acute myeloid leukemia cells (RN2).
创建时间：
2020-07-27
