Divergent responses of soil microbial community after amendment with thermally altered Pinus radiata needles

Post-fire litter layers are composed of leaves and woody debris that predominantly fall during or soon after the fire event. These layers are distinctly different to pre-fire litters due to their common origin and deposition time. However, heterogeneity can arise from the variable thermal conditions in the canopy during fire. Therefore, in this study we used thermally altered pine needles (40, 150, 260, and 320°C) in a laboratory incubation study for 43 days. These samples were measured for respiration throughout and for DNA at the experiment’s end; soil DNA was analysed using Illumina Sequencing (16S and ITS amplicons). Addition of pine needles heated to 40 or 150°C caused a substantial shift in community structure, decreased alpha diversity, and significantly increased soil respiration relative to the control treatment. In contrast, pine needles heated to 260 or 320°C had little effect on microbial community structure or soil respiration. These results indicate that highly thermally altered needles are not microbially decomposed during the first 43 days of exposure and therefore that canopy temperature may have significant effects on post-fire litter decomposition and carbon flux. This research outlines an important knowledge gap in forest fire responses that may affect post-fire carbon emissions estimates.