The amounts of the LO-Bn fusion proteins and the HPLC-purified recombinant Buf IIIb-L monomers obtained from 1×1011 cells of E. coli.
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aTotal protein concentration was determined by BCA protein assay using bovine serum albumin as a standard.bThe amounts of the total expressed LO-Bn fusion proteins and the surface displayed LO-Bn fusion proteins were determined by quantifying the protein bands of SDS-polyacrylamide gels by densitometry at 600 nm as described in Materials and Methods.cThe amounts of the HPLC-purified recombinant Buf IIIb-L monomers were calculated using the calibration curve constructed by plotting the average peak area obtained by HPLC versus the concentration of the synthetic Buf IIIb-L peptide.
创建时间：
2015-12-02
