Presence of CHIKV RNA and infectious CHIKV in the saliva of CHIKV infected cynomolgus macaques.

Monkeys A and B were infected with 106 PFU and monkeys C-F with 4 x 103 PFU of CHIKV. Viral RNA copies were determined by quantitative real time RT-PCR; (the full data set for animals C-F is graphed in Fig C in S1 File. Infectious virus titers were determined by CCID50 assay using BHK-21 cells or PFU assay using Vero cells. Day 2 usually represents peak viraemia [16]. ND—not detected. NT—not tested. 1 Blood noted in sample. Where PFU and CCID50 values were determined, tests were performed at different times with different storage periods.