NGS Data from: Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage
收藏DataCite Commons2025-06-01 更新2025-04-10 收录
下载链接：
https://datadryad.org/dataset/doi:10.5061/dryad.jwstqjqb7
下载链接
链接失效反馈官方服务：
资源简介：
We engineered gRNAs with highly stable hairpins in their constant parts
and further enhanced their stability by chemical modifications. The
‘Genome-editing Optimized Locked Design’ (GOLD)-gRNA increases genome
editing efficiency up to around 1000-fold (from 0.08% to 80.5%) with a
mean increase across different other targets of 7.4-fold. The related NGS
data is deposited here.
提供机构：
Dryad
创建时间：
2022-01-19
