Metabolic capacity of HeLa compared to A549.

Fatty acid profiles were determined by GC-FID analysis of cellular lipid extracts and are expressed as % of total FAME. Data represent means ± SD.aCells were incubated without (−) or with (+) 33 µM of C18∶2n−6 and C18∶3n−3, respectively, for 24 h. Both are substrates for FADS2.bCells were incubated without (−) or with (+) 33 µM of the FADS2 products C18∶3n−6 and C18∶4n−3 for 24 h.cConsidered Δ8-desaturation products.dSynthesis requires upstream a second step of FADS2-encoded activity.eAfter 72 h, significant increase in A459 to 2.7±0.2 when incubated with C18∶3n−3 (FA treatment×cell line: p = .002; see also Figure 1B, peak 14) and to 2.8±0.1 when incubated with C18∶4n−3 (FA treatment×cell line: p = .011).Metabolic capacity of HeLa compared to A549.