Expression factors for oxalate stimulation in HEK293T cells

parental HEK293T were challenged with 1064 µg/cm2 calcium oxalate monohydrate (COM) or sodium oxalate (NaOx) 4mM or vehicle for 24 hours and DNA microarray was performed. We observed and selected two-fold upregulation of laminin, beta 3 (LAMB3), early growth response 1 (EGR1), gremlin 1, DAN family BMP antagonist, Ca++-dependent secretion activator 2, Ras association domain family member 3, interleukin 33 and bone morphogenetic protein 8a , and two-fold down-regulation of interleukin 37 and intercellular adhesion molecule 1 in COM lord compared to vhicle. parental HEK293T were challenged with 1064 µg/cm2 calcium oxalate monohydrate (COM) or sodium oxalate (NaOx) 4mM or vehicle for 24 hours.Total RNA was isolated from cells using RNeasy Plus Mini Kit (QIAGEN) and subjected to microarray data collection at Agilent Technologies Japan, Ltd. (Tokyo Japan) under following conditions.We compared the COM load sample (SAMPLE2; AR3085＿02) with the vhicle sample(SAMPLE1; AR3085＿01).