Main characteristics of the eligible studies sorted by 5 different serum biomarkers.

Note: ELISA* = Enzyme-linked immunosorbent assay; TP* = true positives, FP* = false positives, FN* = false negatives, TN* = true negatives; Abs* = Antibody.
Assay method*：ELISA or ELISA+western = 1; other = 0; unknown = 2.
Formula 1*: normal+2SD
Formula 2*: P53 control protein ratio >1.3
Formula 3*: density>1030
Formula 4*: optical density>0.11
Formula 5*: manufacturer’s formula
Formula 6*: the differences in the absorption values between the positive walls and controls was greater than 0.5
Formula 7*: differences higher than 0.15 were scored positive
Formula 8*: index≥1.1,Absorption> = 1.6
Formula 9*: ≥2.3-times of control
Formula 10*: mean+3SD
Note: Sample collection time*: before diagnosis = 1; before treatment = 0; unknown = 2.
Main characteristics of the eligible studies sorted by 5 different serum biomarkers.