Rlf, a widely-spaced zinc finger protein, is involved in maintaining epigenetic marks at CpG island shores and enhancer elements across the genome

We recently identified a novel protein, Rearranged L-myc fusion (Rlf), that is required for DNA hypomethylation (and transcriptional activity) at certain regions of the mouse genome known to be sensitive to epigenetic gene silencing. Here we report the analysis of 12 whole genome bisulphite sequencing datasets across three different embryonic tissues/stages from mice with and without Rlf. We find that its absence has effects at thousands of differentially methylated regions. Whole genome bilsulphite sequencing, RNAseq and H3K4me1 ChIP-seq for wild type and mice homozygous null for Rlf (MommeD28/MommeD28)我们近期鉴定出一种新型蛋白质——重排L-myc融合蛋白（Rearranged L-myc fusion, Rlf），其在小鼠基因组特定对表观遗传基因沉默敏感的区域中，是DNA低甲基化（及转录活性）维持所必需的。在此，我们报道针对有无Rlf的小鼠的3种不同胚胎组织/发育阶段的12套全基因组亚硫酸氢盐测序（whole genome bisulphite sequencing）数据集的分析结果。研究发现，Rlf的缺失会对数千个差异甲基化区域产生影响。本研究涵盖野生型及Rlf纯合敲除小鼠（MommeD28/MommeD28）的全基因组亚硫酸氢盐测序、RNA测序（RNA-seq）以及组蛋白H3赖氨酸4单甲基化染色质免疫共沉淀测序（H3K4me1 ChIP-seq）相关数据。