RNA-seq profiling of PRAME knockdown in A375SM skin cutaneous melanoma cells

This study investigates the transcriptomic effects of PRAME knockdown in the A375SM skin cutaneous melanoma cell line. Cells were transduced with either control shRNA (shCtrl) or PRAME-targeting shRNA (shPRAME), followed by paired-end RNA sequencing. The dataset provides gene expression changes associated with PRAME silencing and offers insights into PRAME-regulated pathways in melanoma. Overall design: A total of six RNA-seq samples were generated using the A375SM melanoma cell line: three biological replicates transduced with control shRNA (shCtrl) and three replicates transduced with PRAME-targeting shRNA (shPRAME). Total RNA was extracted, polyA-enriched, and sequenced using paired-end 150 bp reads on an Illumina NovaSeq platform. Raw FASTQ files and processed gene expression matrices are provided for differential expression analysis.