IL-36 Amplifies Neutrophilic Lung Inflammation

IL-36, which belongs to the IL-1 superfamily, is increasingly linked to neutrophilic inflammation. Here, we performed single-cell RNA-seq on an acute LPS mouse model of lung inflammation to provide insights into the intercellular signaling pathways and mechanisms through which IL-36 promotes lung inflammation. We identified neutrophils as a source of IL-36 which provides a rationale for targeting IL-36 to improve treatment of a variety of neutrophilic lung diseases. Mice were exposed to aerolized LPS for 4h; for scRNA seq BALF of 8 animals were equally pooled to obtain a single BAL pool that contained the same cell count per animal.