Sustained neurotrophin-3 delivery from hyaluronic acid hydrogels for neural tissue regeneration

The goal of this work was to design a polymer-based platform capable of localized, long-term delivery of biologically active neurotropic factors using an affinity-based approach. Here, we synthesized hyaluronic acid-methylfuran (HA-mF) hydrogels that provide sustained, affinity-based release of neurotrophin-3 (NT-3), a growth factor that promotes axon growth for 28 days. A Diels-Alder crosslinking reaction between HA-mF and polyethylene glycol (PEG)-dimaleimide occurs within 15 minutes under physiological conditions, resulting in injectable hydrogels that can be polymerized in the presence of cells and growth factors. We also tuned the hydrogel’s storage modulus to match that of native rat spinal cord tissue, providing a platform not only for localized drug delivery but also a suitable vehicle for cellular transplantation. The NT-3 released from the HAmF hydrogels remains bioactive for at least 14 days, promoting axonal growth from primary sensory neurons as well as stem cell-derived V2..., Data for NMR was exported as a .fid file.
Storage modulus values were extracted from a rheology time sweep, the values reported are the final stiffness of each run.
Data for protein loading and release experiment was collected using either an ELISA kit or BCA kit and the measurements were converted into concentrations using a standard curve.
Axonal growth data is reported as the normalized average radius extension to the control for each experiment.
PCR data is reported as the ΔΔCt values for each condition and replicate.
Cell viability was measured using a Live/Dead assay kit, values reported are the percentage of live cells for each replicate and condition as measured by fluorescence microscopy and the area quantified., , # Data from: Sustained neurotrophin-3 delivery from hyaluronic acid hydrogels for neural tissue regeneration

Raw data for all figures presented in the paper was collected and compiled into one Excel file (with the exception of the NMR data). For more details regarding the experimental conditions, please refer to the methods section of the manuscript. Information regarding the data collection methods is included below.

## Description of the Data and file structure

Dataset Provided:

Paper Data Excel File

1. NT-3 Loading and equilibrium. NT-3 concentration was measured using a commercially available ELISA kit from R&D Systems. The data was normalized using standard curves. Values reported are:
a) Fraction of NT-3 retained at 24 hours for 10, 50, 100 or 250 ng/mL loading concentrations of NT-3
b) Fraction of NT-3 retained (in) and released (out) for each condition: Phosphate buffer saline (PBS), PBS with 1M sodium chloride (NaCl), or PBS with 2M sodium chloride
2. Storage M...