RNA sequencing of patient derived xenograft sample AML-388 using prime-seq

We establish a Cre-ERT2-loxP (causes recombination, estrogen receptor mutant T2, locus of X-over P1) based inducible RNAi- (ribonucleic acid interference) mediated gene silencing system in patient-derived xenograft (PDX) models of acute leukemias in vivo. Mimicking anti-cancer therapy in patients, gene inhibition is initiated in mice harboring orthotopic tumors. In fluorochrome guided, competitive in vivo trials, silencing of the apoptosis regulator MCL1 (myeloid cell leukemia sequence 1) correlates to pharmacological MCL1 inhibition in patients´ tumors, demonstrating the ability of the method to detect therapeutic vulnerabilities. MCL1 KD and control PDX cells were isolated out of the murine bone marrow after induction of the shRNA expression by tamoxifen. the mRNA expression pattern was analyzed. Please note that the raw data is not provided as the German law does not allow raw sequencing data to be publicly available.