Cancer-cell-secreted miR-9-5p regulates cholesterol homeostasis to promote tumor metastasis [TF TB Rab27AKD miR-9KO]

To investigate the effects of breast cancer derived EVs on liver metabolism,we inoculated MDA-MB-231,231 /Rab27A KD and 231 /miR-9 KO cells into subcutaneous tumor in NSG mice. We then performed gene expression profiling analysis using data obtained from RNA-seq of liver from mice xenografted MDA-MB-231 cells (tumor bearing) or MDA-MB-231/Rab27A KD cells (231/Rab27A KD) or MDA-MB-231 /miR-9 KO (231/miR-9 KO) and tumor free mice. Comparative gene expression profiling analysis of RNA-seq data for liver (tumor free, MDA-MB-231 cells (tumor bearing), MDA-MB-231/Rab27A KD cells (231/Rab27A KD) and MDA-MB-231/miR-9 KO cells (231 /miR-9 KO)).