Mechanisms involved in porcine early embryo survival following ethanol exposure. Sus scrofa

We aimed to determine the toxicogenomic impacts and the mechanisms involved in preimplantation embryonic survival following 0.2% ethanol exposure in porcine embryos. Gene expression changes were measured with a porcine embryo specific microarray and confirmed by RT-qPCR. Compared to control, ethanol exposure led to a 43% decrease in blastocyst rate and activated pathways associated with oxidative stress and nervous system damage, such as TP53 and TGF. Overall design: 4 pools of 10 control porcine blastocysts were compared to 4 pools of 10 ethanol-exposed porcine blastocysts in a microarray experiment.