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Sequence-Targeted Nucleosome Sliding in vivo - Nucleosome Mapping

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https://www.ncbi.nlm.nih.gov/sra/SRP063046
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Nucleosome positions were determined in wild type cells, cells lacking Isw2 or Ume6, and cells containing a hybrid Chd1-Ume6 chimeric remodeler Overall design: Matched MNase digests from W303 strain variants during log growth (OD600=0.4-0.6) were subject to paired-end sequencing for nucleosome mapping. For effects of the engineered fusion remodeling protein, a catalytically inactive (ATPase dead D513N) variant was included as a control.
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2019-09-23
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