Protein profiling of transport vesicles produced under different conditions

1)Identification of cytosolic proteins that are associated with vesicles in a GTP dependent manner and cargo proteins that are packaged into vesicles in a GTP-hydrolysis dependent manner; 2) Identification of cargo proteins and cytosolic proteins that are dependent on Sar1A to be associated with transport vesicles; 3) Identification of cargo proteins that depend on a specific cargo receptor for packaging into transport vesicles