Transcriptomics profiling of white adipose tissue of wildtype (WT) and miR-10a knockout (KO) mice fed a normal chow diet (NCD) or high-fat diet (HFD)

Adipose tissue remodeling is a dynamic process that involves adipocyte death and turnover. This study investigates the influence of miR-10a-5p on the remodeling of adipose tissue and the metabolic dysfunction prompted by a high-fat diet. Our objective involves examining the impact of a 16-week HFD feeding on both WT mice and miR-10a KO mice, comparing the genes that are differentially regulated based on the dietary intake. White adipose tissue mRNA profiles of WT and miR-10a KO mice fed a NCD or HFD were generated by deep sequencing. Three biological replicates were analyzed for each of conditions.脂肪组织重塑（Adipose tissue remodeling）是一个涉及脂肪细胞死亡与更新的动态过程。本研究旨在探究microRNA-10a-5p（miR-10a-5p）对脂肪组织重塑以及高脂饮食诱导的代谢功能紊乱的影响。本研究的核心目标为考察16周高脂饮食（High-Fat Diet, HFD）饲喂对野生型（Wild Type, WT）小鼠及miR-10a敲除小鼠的影响，并对比不同膳食摄入条件下的差异表达基因。本研究通过深度测序技术，获取了正常对照饮食（Normal Control Diet, NCD）或高脂饮食饲喂的野生型小鼠及miR-10a敲除小鼠的白色脂肪组织mRNA表达谱。所有实验条件均设置3次生物学重复并完成分析。