Effect of a naturally eQTN on the yeast transcriptome

We identified a mutation that is a frameshift mutation in a mononucleotide run of eight consecutive T's in the coding region of the gene SSY1, which encodes a key component of a plasma-membrane sensor of extracellular amino acids.We introduced it into the S288c background and examined the effect on gene expression.我们在SSY1基因编码区的一段连续8个胸腺嘧啶（T）的单核苷酸重复序列中，发现了一处移码突变（frameshift mutation）；该基因编码胞外氨基酸质膜传感器的关键组分。随后我们将该突变引入S288c菌株背景，并检测其对基因表达的影响。