Adaptative response of Debaryomyces hansenii in co-culture with Yarrowia lipolytica. Debaryomyces hansenii

A comparison between the coculture of Debaryomyces hansenii with Yarrowia lipolytica and the monoculture of Debaryomyces hansenii, was carried out at two hours of culture (12h and 27h), in order to understand the metabolic changes induced in D. hansenii when grown in coculture with Y. lipolytica . The transcriptomic study was carried out using Agilent 8X15k slides with a custom oligoset designed by V. Loux using the Debaryomyces hansenii ORFeome extracted from genolevures database Overall design: Two conditions experiment, coculture of D. hansenii versus monoculture of D. hansenii at 12h and 27h of culture. Dye switches were done using four biological replicates for both monoculture and coculture of D. hansenii at 12 h and 27h.