Acid boreal peatlands pmoA transcripts Raw sequence reads. peat metagenome

The objective of this study was to characterize metabolically active, aerobic methanotrophs in an ombrotrophic peatland in the Marcell Experimental Forest, Minnesota, USA. Methanotrophs were investigated in the field and in laboratory incubations using DNA-stable isotope probing, expression studies on particulate methane monooxygenase (pmoA) genes, and amplicon sequencing of 16S rRNA genes. In field samples, a transcript-to-gene ratio of 1:2 was observed for pmoA in surface peat layers which attenuated rapidly with depth, indicating the highest methane consumption was associated with the 0-10 cm depth interval. Metagenomes and sequencing of pmoA genes from cDNA confirmed that the dominant active methanotrophs were Methylocystis and Methylomonas.