John Murray (University of Pennsylvania), Ke Hu (Indiana University, Bloomington) (2011) CIL:10508, Toxoplasma gondii RH. CIL. Dataset

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgCentrin2 and mCherry-tagged TgIMC1 (innermembrane complex scaffold protein). Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 100X oil immersion, 1.35 NA. Filters: EGFP, ex 450-490 em 500-540, mCherry, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285.

Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

一层人皮肤成纤维细胞被感染了表达EGFP标记的TgCentrin2和mCherry标记的TgIMC1（内质膜复合体支架蛋白）的弓形虫。使用Olympus IX70倒置显微镜和API DeltaVision系统采集图像。实验目标：采用Olympus 100X油镜，数值孔径为1.35。滤光片：EGFP，激发波长450-490nm，发射波长500-540nm；mCherry，激发波长555-592nm，发射波长600-665nm。二色镜：Chroma 89021。图像采集采用CoolSnapHQ/ICX285设备。该图像为尚未发表的图像，与PMID: 16518471中发表的图像相似。详见出版物获取其他实验细节。