mRNA profiling on TNFR2 knockout CART19 compared to wildtype CART19 produced from normal donors. mRNA profiling on TNFR2 knockout CART19 compared to wildtype CART19 produced from normal donors

RNA sequencing was performed on three biological replicates of untransduced (UTD) T cells, CART19 (TNFR2 wildtype) and TNFR2 knockout CART19 produced from normal donors. Overall design: T cells from normal donors were transduced with a replication-incompetent lentiviral vector expressing a second-generation chimeric antigen receptor (CAR) consisting of an anti-CD19 single chain variable fragment (FMC63) fused to 4-1BB and CD3ζ intracellular domains as previously described. TNFR2 knockout CART19 were also transduced with TNFR2 CRISPR Guide RNA in a pLentiCRISPR v2 backbone as previously described. RNA was isolated on Day 8 of production.