Phenotypes of embryos that were injected with pax6 TALEN mRNAs.

(A) Injection of pax6 TALEN mRNAs to GV oocytes, followed by in vitro maturation and ICSI, recapitulated the null mutant phenotype of pax6 in F0 embryos. Examples of knock-out tadpoles with different magnifications (a-f; pax6 TALEN-expressed tadpoles, g-l; control tadpoles) are shown. The percentages of embryos that showed the knock-out phenotype of pax6 are summarized in the graph, as judged by the degree of eye deformation. Embryos without TALEN mRNA injection are used as a control (Non injected). (B) Different phenotypes are observed at the tadpole stage between conventional embryo injection (Fig 2B) and the oocyte injection method (Fig 2A). TALEN mRNAs were injected into fertilized one-cell stage embryos. Examples of pax6 TALEN-expressed tadpoles with different magnifications (a-d; pax6 TALEN-expressed tadpoles, e-h; control tadpoles) are shown.