Comparative Morphometric Analysis of Corneal Endothelium Before and After Transplantation Using Voronoi Segmentation

This study reports a novel comparative <i>ex vivo</i> and <i>in vivo</i> analysis of the morphometric changes in corneal endothelium pre- and post-transplantation.Images of <i>ex vivo</i> corneal donor tissue pre-transplantation were compared with post-transplant <i>in vivo</i> confocal microscope (IVCM) images of the same tissue. These images were calibrated and analysed using Voronoi segmentation evaluating cell size, shape, and phase, including mean cell area, mean endothelial cell density (ECD), shape area proportion, total number of cells analysed, hexagonal regularity, number of different cell shapes, mean number of cell sides, coefficient of variation (CV) in both shape and size, shape index, and topological charge.