Gene expression of rat PSCs cultured on plastic, matrigel and collagen

Activated pancreatic stellate cells produce the fibrotic matrix in chronic pancreatitis and pancreatic cancer. In vitro protocols examining PSC biology have usually involved PSCs cultured on plastic, a non-physiological surface. However, PSCs cultured on physiological matrices e.g. MatrigelTM (normal basement membrane) and collagen (fibrotic pancreas), may have distinctly different behaviours compared to cells cultured on plastic. Therefore, we aimed to compare PSC gene expression after culture on plastic, MatrigelTM and collagen I.在慢性胰腺炎与胰腺癌的病变进程中，活化胰腺星状细胞（pancreatic stellate cells, PSCs）可产生纤维化基质。既往探究PSC生物学特性的体外研究方案，多将PSC培养于塑料培养板这一非生理表面。然而，与塑料培养板培养的PSC相比，在生理基质（如基质胶（MatrigelTM，正常基底膜）与胶原蛋白（纤维化胰腺来源））中培养的PSC，其生物学行为或存在显著差异。因此本研究旨在对比PSC分别在塑料培养板、基质胶（MatrigelTM）与I型胶原蛋白上培养后的基因表达差异。