OMAP-15 Organ Mapping Antibody Panel (OMAP) for Multiplexed Antibody-Based Imaging of Human Intestines with CODEX, v1.0

OMAP-15 was designed for CO-Detection by indEXing (CODEX), with the Akoya PhenoCycler system for formalin fixed<br>paraffin embedded human intestine samples (https://doi.org/10.1038/s41596-021-00556-8,<br>https://doi.org/10.1002/eji.202048891). The panel contains 54 antibodies and the nuclear marker DAPI. This OMAP<br>provides a spatial context for most anatomical structures and cell types present in the ASCT+B Large Intestine v1.3<br>and Small Intestine v1.1. This OMAP builds from OMAP-2 (https://doi.org/10.48539/HBM244.TNJG.649), adapting the<br>markers for fixed frozen tissues to formalin fixed paraffin embedded (FFPE) tissue. It excludes 19 markers originally<br>found in OMAP-2 and instead includes 22 new markers that include the following: ATP1A1, CD206, COL1A1, CTNNB1, FN1,<br>FOXP3, GP2, HLA-DRA, IGHA1, IGHM, ITGAM, KRT4/5/6a/6b/6c/8/10/13/18, MS4A1, MUC6, PDGFRA/B, POSTN, RORC, SIGLEC8,<br>SPIB, TNC, TPSAB1, and UCHL1. This panel does not contain markers for tuft cells and naive intestinal stem cells<br>(LGR5+ ASCL2+). Antibodies without carriers (BSA) are used and if the regular antibody from the supplier contains<br>carriers, custom ones are ordered without carriers . Antibodies are custom conjugated with oligonucleotide barcodes.<br>The protocols for antibody conjugation (https://doi.org//10.17504/protocols.io.4r3l2q8q4l1y/v1) and sample<br>preparation and antigen retrieval (https://doi.org/10.17504/protocols.io.n92ldm6y9l5b/v1) can be found in the<br>respective linked doi’s. Representative datasets using this OMAP can be found here:<br>https://doi.org/10.5281/zenodo.10963120.