Effects of incubation temperature on the upper thermal tolerance of the imperiled longfin smelt (Spirinchus thaleichthys)

# Effects of incubation temperature on the upper thermal tolerance of the imperiled longfin smelt (Spirinchus thaleichthys) [https://doi.org/10.25338/B8H06D](https://doi.org/10.25338/B8H06D) Contact: Yuzo Yanagitsuru, [e-mail], [e-mail] All analyses were conducted in R version 3.6.3 File: LFS\_cardio\_breakpoint.R includes script that calculated all breakpoint temperatures File: lfs\_cardio\_stats.R includes script for statistical analyses. Description of column headers File: Heart\_rates\_compiled.xlsx 'Analyzed dataset' includes data that were used for analyses. 'with missing measurements' includes data that were incorrectly assayed (temperature steps were missing during assay) * Date - Date assay conducted * ID - Individual fish identification number * Clutch - Clutch/cross identification (i.e. ID for different pairs of male/female broodfish) * Temperature - Temperature in degrees Celsius that heart rates were measured * Acclimation - Incubation temperatures of individuals that were measured * BPM - Heart rate in beats per minute measured * Arrhythmia - Identifier for reached arrhythmia or didn't reach arrhythmia. 0 = not arrhythmic, 1 = arrhythmic * Peaked - Identifier for reached peak heart rate or didn't reach peak heart rate. 0 = not peaked, 1 = peaked * Notes - Visually measured indicates that heart rates were measured by human observation as opposed to Danioscope software. File: Cardiology\_Analysis\_Datasheet.xlsx * ID - individual fish identification number * Acclimation - incubation temperatures of individuals that were measured * Clutch - clutch/cross identification (i.e. id for different pairs of male/female broodfish) * Batch - identification number for grouping of individuals measured together (i.e. fish that have the same batch number were measured at the same time) * Tarr - temperature at which arrhythmia was first observed * Breakpoint - Arrhenius breakpoint temperature, the temperature at which the rate of exponential increase in heart rate changes * Tpeak - temperature at which heart rate was at its maximum * maxhr - heart rate in beats per minute when heart rate was at its maximum * minhr - heart rate in beats per minute when heart rate was at its minimum * Slopes - rate of increase in heart rate prior to reaching Arrhenius breakpoint temperature. Slopes were calculated from plots of Arrhenius temperatures (1/1000\*temperature) vs ln(heart rate) Some values are not available for Tarr and Tab because some individuals did not become arrhythmic and others had a continual increase in heart rate from which Tab could not be determined