Epigenome characterization at single base-pair resolution

We have combined standard micrococcal (MNase) digestion of nuclei with a modified protocol for construction paired-end DNA sequencing libraries to map both nucleosomes and subnucleosome-sized particles at single base-pair resolution throughout the budding yeast genome我们将标准的微球菌核酸酶（micrococcal nuclease, MNase）细胞核消化技术，与改良后的配对末端DNA测序文库构建方案相结合，可在酿酒酵母全基因组范围内实现单碱基分辨率的核小体与亚核小体尺寸颗粒定位分析。