MYC induces oncogenic stress through RNA decay and ribonucleotide catabolism

To investigate the transcriptome that might be dysregulated by MYC hyperactivation specifically when DIS3L is downregulated. MYC was induced for 24 hours by adding 60nM 4-OHT with and without DIS3L depletion in human mammary epithelial cells and rRNA-depleted RNA was sequenced. Total RNA (1ug/sample) was used as input for the NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB). After rRNA depletion, libraries were made using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®, quantified using KAPA Library Quantification Kit, and sequenced on the NextSeq 500/550. After demultiplexing, reads were processed using SAMtools (v1.4) and aligned using Hisat2 (v2.0.4) with default parameters. The reference genome sequences (hg38/GRCh38) and gene annotation files were obtained from the UCSC Genome Browser.