Archival chromatin sequencing in eastern water dragon museum specimens

The data consist of 150 bp paired-end Illumina sequencing reads from chromatin conformation assay of 5 archival eastern water dragon (Intellagama lesueurii lesueurii) specimens and whole genome sequencing of three contemporary specimens. The archival specimens, collected between 1905 and 2001, were spirit-preserved and formalin-exposed. Archival liver tissue was dissected and subjected to MNase treatment heavily optimised for archival tissues. The contemporary specimens had been euthanised due to injury in accordance with Queensland Department of Environment and Sciences permit WA0038029 (Australian Ethics Committee number ANA20161, University of Sunshine Coast). From these fresh specimens, we extracted approximately 5 mg of liver tissue per specimen with the Qiagen AllPrep kit. The extracted or enriched DNA was then prepared for sequencing using an IDT xGEN Prism library prep kit.\n\nThe data were generated as part of a collaboration with Celine Frere at the University of Queensland.