Comparison of different approaches to sequencing low-diversity data or maximizing sequencing accuracy.

Columns: approach name, approach number (for alternative approaches), extra cost ($$$—hundreds of dollars, $$$$—thousands), extra time needed, approach limitations, whether possible after library creation, whether feasible as a rescue strategy, summary recommendation. The cost of the Illumina rehybridization kit is currently ∼$400, cost of lowering cluster density or of mixing with PhiX was estimated from the resulting decreased number of reads from the sample of interest, cost and time necessary for developing new barcodes was estimated based on the need to design and optimize new PCR primers, etc.
Comparison of different approaches to sequencing low-diversity data or maximizing sequencing accuracy.