miR-101-3p represses the migratory and invasive abilities of ovarian cancer cells

This study aimed to assess miR-101-3ps effects on the migratory and invasive abilities of ovarian cancer cells. Cultured ovarian cancer SKOV3 and OVCAR-3 cells were assessed by qRT-PCR for miR-101-3p levels. These cells were assigned to the negative control (NC) and miR-101-3p mimic groups, to undergo transfection with empty vector and miR-101-3p mimic, respectively. Cell viability analysis employed CCK8 at 0, 24, 48, and 72 h. The migratory potential of cells was quantitated by the wound healing assay at 0, 24, and 48 h, while the Transwell assay was employed to asses invasion at 24 h. The cells underwent mRNA high-throughput sequencing, and qPCR was carried out for the confirmation of data for three mRNAs. After miR-101-3p mimic transfection, miR-101-3p levels were starkly increased in SKOV3 and OVCAR-3 cells in comparison with the respective NC groups. In addition, the miR-101-3p mimic groups displayed remarkably decreased cell viability at 24, 48, and 72 h versus the respective NC groups; meanwhile, the migratory and invasive abilities were lowered in cells administered miR-101-3p mimic. mRNA high-throughput sequencing identified differentially expressed genes, including FN-1 and CXCL8, and qRT-PCR validation data were consistent. miR-101-3p inhibits migration and invasion in ovarian cancer cell lines.