Chromatin accesibility characteristics of human primary, bone marrow-derived and iPSC-derived monocytes and macrophages [ATAC-Seq]

In vitro models to study diseases often involve human primary monocytes which are subjected to a interpersonal variability and short half-life. Bone marrow and iPSC-derived monocytes as an alternative for these in vitro models. However, the similarities and differences of these cells compared to human circulating monocytes has not yet been described. In this study, we compared human primary circulating, bone marrow-derived and iPSC-derived monocytes and macrophages and its functional, epigenomic, transcriptomic and morpholoigcal lanscape. Overall design: We isolated human circulating monocytes from healthy donors, generated iPSC-derived monocytes from iPSCs, and differentiated monocytes derived from BM aspirates to compare morphology, subsets, functionality, metabolic pathways, gene expression and gene accessibility, and polarization towards macrophages. 50.000 monocytes or macrophages were using TDE1 Tagment DNA Enzyme, TD buffer (Illumina Tagment DNA TDE1 Enzyme Buffer), and 1% Digitonin (Promega, G9441)